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Antibody-mediated reversal of peptide-induced inhibition of DPP3 activity. ADM (panel A), ADM1–14 (panel B), and ADM-Gly (panel C), respectively, was added at a constant final concentration of 10 µM in the DPP3 activity assay, and increasing amounts of the anti-N-terminal ADM antibody and the negative control antibody Palivizumab, respectively, were added at the indicated concentrations. The inhibition achieved with 10 µM of each peptide was defined as 100% inhibition, and inhibition measured in the presence of antibodies was related to this. The maximal reversal of peptide-induced inhibition of DPP3 activity was defined as 0% inhibition. Shown are mean (SD) values gained from duplicate measurements. For all three peptides, the reversal of peptide-induced inhibition of DPP3 activity by the anti-ADM antibody was statistically significant compared to the negative control antibody Palivizumab ( p > 0.05 by ANOVA).

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Influence of adrenomedullin on the enzymatic activity of dipeptidyl peptidase 3 in vitro

doi: 10.1080/14756366.2026.2700037

Figure Lengend Snippet: Antibody-mediated reversal of peptide-induced inhibition of DPP3 activity. ADM (panel A), ADM1–14 (panel B), and ADM-Gly (panel C), respectively, was added at a constant final concentration of 10 µM in the DPP3 activity assay, and increasing amounts of the anti-N-terminal ADM antibody and the negative control antibody Palivizumab, respectively, were added at the indicated concentrations. The inhibition achieved with 10 µM of each peptide was defined as 100% inhibition, and inhibition measured in the presence of antibodies was related to this. The maximal reversal of peptide-induced inhibition of DPP3 activity was defined as 0% inhibition. Shown are mean (SD) values gained from duplicate measurements. For all three peptides, the reversal of peptide-induced inhibition of DPP3 activity by the anti-ADM antibody was statistically significant compared to the negative control antibody Palivizumab ( p > 0.05 by ANOVA).

Article Snippet: A humanised anti-N-terminal ADM monoclonal antibody (initially named Adrecizumab, later Enibarcimab) was purchased from (MedChemExpress, Monmouth Junction, USA).

Techniques: Inhibition, Activity Assay, Concentration Assay, Negative Control